Milk vs. Blood - Which is Best for PAG Pregnancy Prediction?
نویسندگان
چکیده
Identification of nonpregnant dairy cows early after AI improves reproductive efficiency and the 21-day pregnancy rate by decreasing the interval between AI services thereby increasing the AI service rate (Fricke, 2002). Thus, new technologies to identify nonpregnant dairy cows early after AI may play a key role in management strategies to improve reproductive efficiency and profitability on dairy farms. Assays for detecting pregnancy-associated glycoprotein (PAG) levels in maternal circulation originating from mononucleated and binucleated cells of the embryonic trophoblast have been developed and commercialized to determine pregnancy status in cattle (Sasser et al., 1986; Zoli et al., 1992; Green et al., 2000).
منابع مشابه
Factors associated with pregnancy-associated glycoprotein (PAG) levels in plasma and milk of Holstein cows during early pregnancy and their effect on the accuracy of pregnancy diagnosis.
Lactating Holstein cows (n = 141) were synchronized to receive their first timed artificial insemination (TAI). Blood and milk samples were collected 25 and 32 d after TAI, and pregnancy status was determined 32 d after TAI using transrectal ultrasonography. Cows diagnosed pregnant with singletons (n = 48) continued the experiment in which blood and milk samples were collected and pregnancy sta...
متن کاملPregnancy-associated glycoproteins (PAG) as pregnancy markers in the ruminants.
In the last years, a polymorphic family of placenta-expressed proteins has been discovered in ruminant species and used for pregnancy diagnosis. Pregnancy diagnosis is an important part in reproduction management of ruminants. The pregnancy-associated glycoproteins (PAG) are synthesized in the mono- and binucleate cells of the ruminant's trophectoderm. Part of them is released into maternal blo...
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This study was designed to establish possible factors affecting plasma pregnancy-associated glycoprotein (PAG) concentrations during early pregnancy in high producing dairy cows with live fetuses. Blood samples were obtained on days 35, 42, 49, 56 and 63 of gestation from 80 lactating cows in two herds carrying live fetuses. Radioimmunoassay systems were used to determine PAG (RIA-497 and RIA-7...
متن کاملNegative Prediction Value of Pregnancy-associated Glycoprotein Contributes to Reduce the Days During Which Nonpregnant Holstein Cows are Subjected to Diverse Strategies of Hormonal Synchronization
The objective of the present study was to determine the accuracy and the negative prediction value of Pregnancy-Associated Glycoprotein (PAG) at 29 d post artificial insemination by ELISA. Blood serums of multiparous Holstein cows were used (n = 275). Pregnancy diagnosis was carried out by rectal palpation at 40 d and it was considered as reference test. Pregnancy results based on the PAG-ELISA...
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Differences in placental mass and vascularity exist between cows gestating single vs. multiple fetuses. Therefore, the association between fetal number and placental development or function was assessed by comparing concentrations of vascular endothelial growth factor (VEGF), pregnancy-associated glycoproteins (PAG), IGF-I, and progesterone in the maternal blood of cattle selected for twin birt...
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